A collaboration between the Yokoyama and Yang Labs has led to a publication of their latest work on the catalytic mechanism of MoaA radical SAM cyclase in molybdenum cofactor biosynthesis. In this work, combined efforts using electrochemistry, EPR spectroscopy, and DFT calculation provided evidence that the reduction of the aminyl radical intermediate proceeds by a proton-coupled electron transfer (PCET) with a 4Fe-4S cluster as an electron donor. The EPR also revealed an electronic coupling between GTP and the 4Fe-4S cluster, suggesting a potentially missing mechanism of catalysis. Read more about their findings in a recent JACS, available here.
Chemistry
