Abstract: One goal of our group is to design and implement high-throughput screening (HTS) assays for enantiomeric excess (ee) in catalytic asymmetric reaction screening. The analysis is performed in microtiter plates where the ee values, as well as concentration (reaction yield), of 96 crude reaction mixtures. Assays for diols, amines, carboxylic acids, ketones, and alcohols have been created.
Mechanistic chemistry can be utilized to generate signal amplification in sensing routines. We have recently exploited auto-inductive cascades to measure the biproducts of hydrolysis of nerve agents. In this tale, the concepts and approaches will be delineated to detect fluoride and thiols, the products from V- and G- agents.
The senses of taste and smell operate via a series of cross-reactive protein-based receptors that are non-selective, but create patterns that discriminate solution and vapor composition, respectively. It will be shown that a union of designed receptors targeted to a class of analytes, with combinatorial methods, gives fingerprints that differentiate between the individual members of the class. The technique represents a marriage of supramolecular chemistry and chemometrics.