Pushing the Spatiotemporal Limits of Optical Microscopy to Explore Real-Time Cellular Dynamics
Courtney Johnson, graduate student
Kevin Welsher, advisor
Friday, April 21, 2017 - 1:00pm to 2:00pm
Location: French Family Science Center 3232
Avery, Meg


Development of the optical microscopy method 3D Multi-Resolution Microscopy combines the robust single particle tracking capability of 3D-Dynamic Photon Localization Tracking (3D-DyPLOT) with the optical sectioning power of Two-Photon Laser Scanning Microscopy (2P-LSM) and an electrically-tunable lens (ETL) to image the three-dimensional environment surrounding the locked-on particle. This resulting 3D image provides important biological context about the location and activity of the particle being tracked. This method will allow for the exploration of high-speed interactions between nanoparticles and larger structures at 3D interfaces.